Ipaliwanag sa Tagalog at magbigay ng halimbawa
Positive Result: Dark brown rhombic crystal of haemin or haematin chloride arranged singly or in cluster.
Limitation of the Test: The test is also given by indigo-dyed fabrics. If the stain is old or washed or is changed by chemical reagents, the crystals are not formed. The addition of too much salt or presence or moisture in the acid or over-heating of the slide may result in failure.
b. THE ACETONE-HAEMIN TEST
The test depends on the addition of specific chemicals to the blood so that characteristic crystal with hemoglobin will be formed.
Reagent: Acetone, dilute acetic acid or oxalic acid
Procedure: Place dried stain on a glass slide and cover with cover slip with a needle interposed to prevent direct contact of the cover slip with the slide. Add a drop of acetone then a drop of acetic acid.
Positive Result: Small dark, diachronic acicular crystals of acetone haemin.
c. THE HAEMOCHROMOGEN CRYSTAL TEST OF THE TAKAYAMA TEST
A delicate test for the presence of hemoglobin. The test depends on the addition of specific chemicals to the blood so that characteristic crystals of hemoglobin derivatives will be formed.
Reagent: Takayama reagent (3 ml of 10% sodium hydroxide, 33 ml of pyridine, 3 ce
of saturated glucose solution and diluted with 7 ml water)
Procedure: Place a small piece of suspected material on a glass slide. Add a drop of takayama reagent. Cover with a glass slip.
Positive Result:, Large rhombic crystals of a salmon pink color arranged in cluster, sheaves and other forms that appear within 1 to 6 minutes when viewed under the low power objective. To hasten result heat may be applied.
C. THE SPECTROSCOPE TEST FOR BLOOD
The most delicate and reliable test for the determination of the presence of blood both old and recent stains. This test is performed by means of an optical
Positive Result: Dark brown rhombic crystal of haemin or haematin chloride arranged singly or in cluster.
Limitation of the Test: The test is also given by indigo-dyed fabrics. If the stain is old or washed or is changed by chemical reagents, the crystals are not formed. The addition of too much salt or presence or moisture in the acid or over-heating of the slide may result in failure.
b. THE ACETONE-HAEMIN TEST
The test depends on the addition of specific chemicals to the blood so that characteristic crystal with hemoglobin will be formed.
Reagent: Acetone, dilute acetic acid or oxalic acid
Procedure: Place dried stain on a glass slide and cover with cover slip with a needle interposed to prevent direct contact of the cover slip with the slide. Add a drop of acetone then a drop of acetic acid.
Positive Result: Small dark, diachronic acicular crystals of acetone haemin.
c. THE HAEMOCHROMOGEN CRYSTAL TEST OF THE TAKAYAMA TEST
A delicate test for the presence of hemoglobin. The test depends on the addition of specific chemicals to the blood so that characteristic crystals of hemoglobin derivatives will be formed.
Reagent: Takayama reagent (3 ml of 10% sodium hydroxide, 33 ml of pyridine, 3 ce
of saturated glucose solution and diluted with 7 ml water)
Procedure: Place a small piece of suspected material on a glass slide. Add a drop of takayama reagent. Cover with a glass slip.
Positive Result:, Large rhombic crystals of a salmon pink color arranged in cluster, sheaves and other forms that appear within 1 to 6 minutes when viewed under the low power objective. To hasten result heat may be applied.
C. THE SPECTROSCOPE TEST FOR BLOOD
The most delicate and reliable test for the determination of the presence of blood both old and recent stains. This test is performed by means of an optical