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a. Fix preparation in absolute methyl ******* for 3 minutes. Stain in a 0.5% aqueous solution of eosin for 1-3 minutes Loffer's methylene blue is added for 1-3 minutes. Sosin stains the red blood cells, whille méthylene blue stains the nuclel
b. Fix smear with methyl or ethyl ******* for 3 minutes. Pour off ******* and flood smear with Gelmaa's stain. Stain for 15 minutes, cover to prevent evaporation, wash in water and dry.
c. Wright's Stain- The smear is flooded with the stain and allowed to stand for a minute. Distilled water is added until a metallic scum forms on the surface. Let stand for 3 minutes, wash with water anddry.
Visible Results:
1. Mammalian red blood cells - circular, biconcave discs with nucleus. Appear as characteristics non-nucleated discs. Exception is camel and closely related animal as llama whose red blood cells are oval but also without nucleus
2. Birds, fish and reptile red blood cells - larges, oval and nucleated.
3. Amphibian red blood cells - are larger than mammals, oval and nucleated.
4. Lamprey eel red blood cells - circular and nucleated.
B. THE MICROCHEMICAL TEST AND MICROCRYSTALLINE TEST FOR BLOOD
'The identification of blood can be made more specific if microchemical or microcrystalline test is applied or performed. Takayama test and Teicmann test are the most popular ones.
The Three Microchemical and Microcrystalline Test for Blood 1. Teicmann Haemin Reaction or Teicmann Test or Haemin Crystal Test
2. Acetone-Haemin Test
3. Haemochromogen Crystal Test or Takayama Test
a. THE TEICMANN TEST
The test depends on the addition of the specific chemicals to the blood so that characteristics crystals with hemoglobin will be formed.
geat: Sodium chloride, glacial acetic acid
Procedure: Place, a minute fragment of the stain on a glass slide. Add a sal Crystal of sodium chloride and 2 to 3 drops of acetic acid. Place cover slip and heat Bently over small flame to evaporate the acid. Cool. Examine under the high power objective.
a. Fix preparation in absolute methyl ******* for 3 minutes. Stain in a 0.5% aqueous solution of eosin for 1-3 minutes Loffer's methylene blue is added for 1-3 minutes. Sosin stains the red blood cells, whille méthylene blue stains the nuclel
b. Fix smear with methyl or ethyl ******* for 3 minutes. Pour off ******* and flood smear with Gelmaa's stain. Stain for 15 minutes, cover to prevent evaporation, wash in water and dry.
c. Wright's Stain- The smear is flooded with the stain and allowed to stand for a minute. Distilled water is added until a metallic scum forms on the surface. Let stand for 3 minutes, wash with water anddry.
Visible Results:
1. Mammalian red blood cells - circular, biconcave discs with nucleus. Appear as characteristics non-nucleated discs. Exception is camel and closely related animal as llama whose red blood cells are oval but also without nucleus
2. Birds, fish and reptile red blood cells - larges, oval and nucleated.
3. Amphibian red blood cells - are larger than mammals, oval and nucleated.
4. Lamprey eel red blood cells - circular and nucleated.
B. THE MICROCHEMICAL TEST AND MICROCRYSTALLINE TEST FOR BLOOD
'The identification of blood can be made more specific if microchemical or microcrystalline test is applied or performed. Takayama test and Teicmann test are the most popular ones.
The Three Microchemical and Microcrystalline Test for Blood 1. Teicmann Haemin Reaction or Teicmann Test or Haemin Crystal Test
2. Acetone-Haemin Test
3. Haemochromogen Crystal Test or Takayama Test
a. THE TEICMANN TEST
The test depends on the addition of the specific chemicals to the blood so that characteristics crystals with hemoglobin will be formed.
geat: Sodium chloride, glacial acetic acid
Procedure: Place, a minute fragment of the stain on a glass slide. Add a sal Crystal of sodium chloride and 2 to 3 drops of acetic acid. Place cover slip and heat Bently over small flame to evaporate the acid. Cool. Examine under the high power objective.