A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.
These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction digestion; meanwhile, host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.More than 3,600 restriction endonucleases are known which represent over 250 different specificities. Over 3,000 of these have been studied in detail, and more than 800 of these are available commercially. These enzymes are routinely used for DNA modification in laboratories, and they are a vital tool in molecular cloning.
Mga master sa mga nagme- maintain ng Messenger app Group Chat (GC) as Admin. Meron po bang region restriction ang Messenger app? May GC po kami (isa po ako sa admin) consist of members located sa Pilipinas at meron sa iba't-ibang bansa, di ko po magawa and mga sumusunod:
1. Gamitin yung...
Tanong lang mga maam/sir, pag po ba mag dagdag ako ng restriction sa DL ko. need ko ba ulit kumuha ng students permit or diretso na po practical at theoretical?
Currently pang motor lang meron ako, plan ko sana magdagdag ng pang sasakyan. Salamat po
Hello po , ask ko lang sana gustu ko sana kumuha Ng motor na Honda RS 125,
Pero Yung lisensya ko Po ay may Code A (AT) pwede ba ako mag maneho Ng Honda RS 125?
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Guys i need your help sa mga may alam kasi plano ko mag paadd ng Restriction Code for 4wheels. Automatic na Motor lang kasi ang meron sa lisensya ko tapos gusto ko isama na yung para sa mga van at close van.
Dun sa napagtanungan ko na driving instructor e to add B1 at B2 need daw nila ng copy...
Hello po sa mga kapwa ko motorista at mga kaphcorner!
Ano po ang mga requirements para sa additional restrictions? Mag paadd sana ako ng 4wheels na manual.
Ano ano din po ang tips nyo sa computer exam at lalo na sa practical driving exam?