A transfer DNA (T-DNA) binary system is a pair of plasmids consisting of a T-DNA binary vector and a vir helper plasmid. The two plasmids are used together (thus binary) to produce genetically modified plants. They are artificial vectors that have been derived from the naturally occurring Ti plasmid found in bacterial species of the genus Agrobacterium, such as A. tumefaciens. The binary vector is a shuttle vector, so-called because it is able to replicate in multiple hosts (e.g. Escherichia coli and Agrobacterium).
Systems in which T-DNA and vir genes are located on separate replicons are called T-DNA binary systems. T-DNA is located on the binary vector (the non-T-DNA region of this vector containing origin(s) of replication that could function both in E. coli and Agrobacterium, and antibiotic resistance genes used to select for the presence of the binary vector in bacteria, became known as vector backbone sequences). The replicon containing the vir genes became known as the vir helper plasmid. The vir helper plasmid is considered disarmed if it does not contain oncogenes that could be transferred to a plant.
Mga sir. Patulong naman po. Dami kk na natry na vpn. Pero bakit po ganun lahat bumibitaw sa download. Sa una speed ko us up to 30mbps downspeed niya tas bigla baba ng baba hanggang wala na. Ginamitan ko na rin ng l4d ping tool pero bumibitaw pa rin. Patulong naman po.
Hello po.. yung sa magaling mag edit sa Amaze VPN
ano po problem neto?
/data/data/com.gmail.heagoo.apkeditor.pro/files/decoded/AndroidManifest.xml:22: error: No resource identifier found for attribute 'qihoo' in package 'android'
Bakit po pag nag injector ako pag connected na tapos nag laro po ako ng mobile legends wala na po ung net . si injector lumalabas at nawawala po ung susi pa help naman po any suggestion po ?
Mga TS sorry na nag post ako ng hindi talaga pwidi pero need na talaga kasi malapit na maobos FREE 100mb ko sa smart 3mb nalang kindly send nio naman new Ps sa sun PLss.. Sana my magandang loob mg pm
patulong naman po ...paano po ba mag-häçk ng internet any suggestion lang po para din makatulong ako sa kapwa ka-PH.....Saan po ba ako magsisimula....?willing po akong matuto kahit mahirap...
Guys bkt gnun .
Sun user ako .
Nwala h+ ko
But still nkkpg injector ako
My problem is
Pg 3g ko. Wala na nklgay na h+
Knina meron
Pg 2g nmn . lumabas G . LNG
Tpos pg gnwa ko na 3g . browsing LNG . dnko nkk receive ng text
Unless. Gwn ko 2g .mkka receive ako bkt nging gnun
Help
Mga Boss panu kaya ma open si 936 ayaw pumasok nung user at passwrd nya .
User:admin
Pass:admin
Yan yun ayaw nya gumana eh hindi ko naman ginagalaw yan :( .
good day po sa inyo pahelp nm po regarding sa quencee vpn
problem po ay bumabagal po ksi sya after mga 2 mins and nwawala po ung internet pero connected nmn po
thanks and more power to u guys
Need ko po ng serious na sagot....
Ano po ba pwede isampang kaso sa isang tao na mahilig magkwento pero hindi nmn po dapat ipagkalat sa public yung nakita niya kasi personal po iyon..
Gusto kasi ng kaibigan ko na mabigyan leksyon yung taong yun
Salamat po
Tulong naman po . ahm nung isang araw gumamit po ako ng ponpon sa pocket wifi . kaso hnd sya gumana sa tingin ko po kasi hnd sya pang pocket . tas pinatay ko yung pocket wifi at natulog ako . Then pag gising ko sa umaga binuksan ko ulit ang pocket wifi . Meron po ako isang ponpon na komo...
need ko po ng help gusto ko po kc maupdate ung alcatel one touch po c7 ko na jelly bean in to kitkat android version possible nman daw po ung kaso di ko alam kung paano.... i need ung help any one who can help me?